The toxicity of ZnO and CuO nanoparticles on biological wastewater treatment and its detoxification: a review / 生物工程学报

The wide use of ZnO and CuO nanoparticles in research, medicine, industry, and other fields has raised concerns about their biosafety. It is therefore unavoidable to be discharged into the sewage treatment system. Due to the unique physical and chemical properties of ZnO NPs and CuO NPs, it may be toxic to the members of the microbial community and their growth and metabolism, which in turn affects the stable operation of sewage nitrogen removal. This study summarizes the toxicity mechanism of two typical metal oxide nanoparticles (ZnO NPs and CuO NPs) to nitrogen removal microorganisms in sewage treatment systems. Furthermore, the factors affecting the cytotoxicity of metal oxide nanoparticles (MONPs) are summarized. This review aims to provide a theoretical basis and support for the future mitigating and emergent treatment of the adverse effects of nanoparticles on sewage treatment systems.

Degradation of Caffeine by Heterogeneous Photocatalysis Using ZnO with Fe and Ag

Abstract The organic compound caffeine when detected in environmental matrices such as surface waters and groundwater is considered as an emerging contaminant, in which its effects are still unknown. Therefore, in the present research, zinc oxide-based catalysts impregnated with iron and silver were prepared for the reaction of caffeine degradation by heterogeneous photocatalysis. The wet impregnation method with excess solvent was applied to the preparation of the materials, later they were characterized by adsorption of N2, X-ray diffraction and photoacoustic spectroscopy. Then, the photodegradation, photolysis and adsorption tests were carried out, in which it was observed that only the presence of the radiation or photocatalysts could not sufficiently degrade the caffeine, however when combined radiation with all the catalysts studied here presented degradation above 70% at the end of 300 minutes of the reaction, and the best catalyst studied was that containing 8% Ag in non-calcined ZnO. Thus, these results point out that the methodology employed in this research, both for the preparation of the catalysts and in the process of the photocatalysis reaction, was efficient in the degradation of the emerging contaminant, caffeine, which could later be used for a mixture of other contaminants.

Impact of calcium aluminate cement with additives on dental pulp-derived cells

Abstract Calcium aluminate cement (CAC) has been highlighted as a promising alternative for endodontic use aiming at periapical tissue repair. However, its effects on dental pulp cells have been poorly explored. Objective: This study assessed the impact of calcium chloride (CaCl2) and bismuth oxide (Bi2O3) or zinc oxide (ZnO) additives on odontoblast cell response to CAC. Methodology: MDPC-23 cells were exposed for up to 14 d: 1) CAC with 2.8% CaCl2 and 25% ZnO (CACz); 2) CAC with 2.8% CaCl2 and 25% Bi2O3 (CACb); 3) CAC with 10% CaCl2 and 25% Bi2O3 (CACb+); or 4) mineral trioxide aggregate (MTA), placed on inserts. Non-exposed cultures served as control. Cell morphology, cell viability, gene expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), and dentin matrix protein 1 (DMP-1), ALP activity, and extracellular matrix mineralization were evaluated. Data were compared using ANOVA (α=5%). Results: Lower cell density was detected only for MTA and CACb+ compared with Control, with areas showing reduced cell spreading. Cell viability was similar among groups at days one and three (p>0.05). CACb+ and MTA showed the lowest cell viability values at day seven (p>0.05). CACb and CACb+ promoted higher ALP and BSP expression compared with CACz (p<0.05); despite that, all cements supported ALP activity. Matrix mineralization were enhanced in CACb+ and MTA. Conclusion: In conclusion, CAC with Bi2O3, but not with ZnO, supported the expression of odontoblastic phenotype, but only the composition with 10% CaCl2 promoted mineralized matrix formation, rendering it suitable for dentin-pulp complex repair.

Influence of chlorhexidine and zinc oxide in calcium hydroxide pastes on pH changes in external root surface

Abstract The objective of this study was to assess the influence of chlorhexidine (liquid and gel) and zinc oxide in calcium hydroxide (CH) pastes on root pH in simulated external resorption. One hundred human anterior teeth with a single root canal were selected. After decoronation and root canal instrumentation, the specimens were divided into 4 experimental groups and 1 control group (without intracanal paste): CH + saline (CH+S), CH + 2% chlorhexidine liquid (CH+ CHX), CH + 2% chlorhexidine gel (CH+ CHXg), and CH + 2% chlorhexidine gel + zinc oxide (CH+ CHXg+ZnO). pH was measured using a microelectrode at 3 and 24 h, and 1, 2, 3, and 4 weeks after inserting intracanal pastes. Data were analyzed statistically using an ANOVA and Tukey's test (p < 0.05). The CH+CHXg+ZnO group had the highest pH values throughout (p<0.05). The CH+S and CH+ CHX groups had the highest pH values after 1 week and the CH+ CHXg group after 2 weeks. CH+ CHXg maintained the highest pH until the fourth week compared with CH+ CHX (p < 0.05). The control group remained at a neutral pH at all evaluated times. It can be concluded that chlorhexidine solution or gel maintained the alkaline pH of CH, and chlorhexidine gel allowed a slower decrease in pH over time. CH+ CHXg+ZnO showed the highest pH values and was an effective intracanal medication for maintaining alkaline root pH in the area of resorption.

Model resin composites incorporating ZnO-NP: activity against S. mutans and physicochemical properties characterization

Abstract Although resin composites are widely used in the clinical practice, the development of recurrent caries at composite-tooth interface still remains as one of the principal shortcomings to be overcome in this field. Objectives To evaluate the activity against S. mutans biofilm of model resin composites incorporating different concentrations of ZnO-nanoparticles (ZnO-NP) and characterize their physicochemical properties. Materials and Methods Different concentrations of ZnO-NP (wt.%): E1=0, E2=0.5, E3=1, E4=2, E5=5 and E6=10 were incorporated into a model resin composite consisting of Bis-GMA-TEGDMA and barium borosilicate particles. The activity against S. mutans biofilm was evaluated by metabolic activity and lactic acid production. The following physicochemical properties were characterized: degree of conversion (DC%), flexural strength (FS), elastic modulus (EM), hardness (KHN), water sorption (Wsp), water solubility (Wsl) and translucency (TP). Results E3, E4, E5 and E6 decreased the biofilm metabolic activity and E5 and E6 decreased the lactic acid production (p<0.05). E6 presented the lowest DC% (p<0.05). No significant difference in FS and EM was found for all resin composites (p>0.05). E5 and E6 presented the lowest values of KHN (p<0.05). E6 presented a higher Wsp than E1 (p<0.05) and the highest Wsl (p<0.05). The translucency significantly decreased as the ZnO- NP concentration increased (p<0.05). Conclusions The incorporation of 2 - 5 wt.% of ZnO-NP could endow antibacterial activity to resin composites, without jeopardizing their physicochemical properties.

Molecular and morphological surface analysis: effect of filling pastes and cleaning agents on root dentin

Abstract The quality of the dentin root is the most important factor for restoration resin sealing and drives the outcome of endodontic treatment. Objective This study evaluated the effect of different filling pastes and cleaning agents on the root dentin of primary teeth using Fourier-transformed Raman spectroscopy (FT-Raman), micro energy-dispersive X-ray fluorescence (µ-EDXRF) and scanning electron microscopic (SEM) analysis. Material and Methods Eighty roots of primary teeth were endodontically prepared and distributed into 4 groups and filled according to the following filling pastes: Control-no filling (CP), Calen®+zinc oxide (CZ), Calcipex II® (CII), Vitapex® (V). After seven days, filling paste groups were distributed to 4 subgroups according to cleaning agents (n=5): Control-no cleaning (C), Ethanol (E), Tergenform® (T), 35% Phosphoric acid (PA). Then, the roots were sectioned and the dentin root sections were internally evaluated by FT-Raman, µ-EDXRF and SEM. Data was submitted to two-way ANOVA and Tukey tests (α=0.05). Results Regarding filling pastes, there was no significant difference in organic content. CP provided the lowest calcium values and, calcium/phosphoric ratio (Ca/P), and the highest phosphoric values. For cleaning agents there was no difference in organic content when compared to the C; however, T showed significantly higher calcium and Ca/P than PA. All groups showed similar results for phosphorus. The dentin smear layer was present after use of the cleaning agents, except PA. Conclusion The filling pastes changed the inorganic content, however they did not change the organic content. Cleaning agents did not alter the inorganic and organic content. PA cleaned and opened dentin tubules.

Solubility and bacterial sealing ability of MTA and root-end filling materials

ABSTRACT Objective To evaluate solubility and sealing ability of Mineral Trioxide Aggregate (MTA) and root-end filling materials. Material and Methods The materials evaluated were: MTA, Calcium Silicate Cement with zirconium oxide (CSC/ZrO2), and zinc oxide/eugenol (ZOE). Solubility test was performed according to ANSI/ADA. The difference between initial and final mass of the materials was analyzed after immersion in distilled water for 7 and 30 days. Retrograde cavities in human teeth with single straight root canal were performed by using ultrasonic tip CVD 9.5107-8. The cavities were filled with the evaluated materials to evaluate sealing ability using the bacterial leakage test with Enterococcus faecalis. Bacterial leakage was evaluated every 24 hours for six weeks observing the turbidity of Brain Heart infusion (BHI) medium in contact with root apex. Data were submitted to ANOVA followed by Tukey tests (solubility), and Kruskal-Wallis and Dunn tests (sealing ability) at a 5% significance level. Results For the 7-day period, ZOE presented highest solubility when compared with the other groups (p<0.05). For the 30-day period, no difference was observed among the materials. Lower bacterial leakage was observed for MTA and CSC/ZrO2, and both presented better results than ZOE (p<0.05). Conclusion MTA and CSC/ZrO2 presented better bacterial sealing capacity, which may be related to lower initial solubility observed for these materials in relation to ZOE.

Biological and mechanical properties of an experimental glass-ionomer cement modified by partial replacement of CaO with MgO or ZnO

AbstractSome weaknesses of conventional glass ionomer cement (GIC) as dental materials, for instance the lack of bioactive potential and poor mechanical properties, remain unsolved.Objective The purpose of this study was to investigate the effects of the partial replacement of CaO with MgO or ZnO on the mechanical and biological properties of the experimental glass ionomer cements.Material and Methods Calcium fluoro-alumino-silicate glass was prepared for an experimental glass ionomer cement by melt quenching technique. The glass composition was modified by partial replacement (10 mol%) of CaO with MgO or ZnO. Net setting time, compressive and flexural properties, and in vitrorat dental pulp stem cells (rDPSCs) viability were examined for the prepared GICs and compared to a commercial GIC.Results The experimental GICs set more slowly than the commercial product, but their extended setting times are still within the maximum limit (8 min) specified in ISO 9917-1. Compressive strength of the experimental GIC was not increased by the partial substitution of CaO with either MgO or ZnO, but was comparable to the commercial control. For flexural properties, although there was no significance between the base and the modified glass, all prepared GICs marked a statistically higher flexural strength (p<0.05) and comparable modulus to control. The modified cements showed increased cell viability for rDPSCs.Conclusions The experimental GICs modified with MgO or ZnO can be considered bioactive dental materials.

Push-out bond strength of fiber posts to root dentin using glass ionomer and resin modified glass ionomer cements

OBJECTIVE: The purpose of this study was to assess the push-out bond strength of glass fiber posts to root dentin after cementation with glass ionomer (GICs) and resinmodified glass ionomer cements (RMGICs). MATERIAL AND METHODS: Fifty human maxillary canines were transversally sectioned at 15 mm from the apex. Canals were prepared with a step back technique until the application of a #55 K-file and filled. Post spaces were prepared and specimens were divided into five groups according to the cement used for post cementation: Luting & Lining Cement; Fuji II LC Improved; RelyX Luting; Ketac Cem; and Ionoseal. After cementation of the glass fiber posts, all roots were stored at 100% humidity until testing. For push-out test, 1-mm thick slices were produced. The push-out test was performed in a universal testing machine at a crosshead speed of 0.5 mm/minute and the values (MPa) were analyzed by Kolmogorov-Smirnov and Levene's tests and by two-way ANOVA and Tukey's post hoc test at a significance level of 5%. RESULTS: Fiber posts cemented using Luting & Lining Cement, Fuji II LC Improved, and Ketac Cem presented the highest bond strength to root dentin, followed by RelyX Luting. Ionoseal presented the lowest bond strength values (P>0.05). The post level did not influence the bond strength of fiber posts to root dentin (P=0.148). The major cause of failure was cohesive at the cement for all GICs and RMGICs. CONCLUSIONS: Except for Ionoseal, all cements provided satisfactory bond strength values. .

Efecto de la humedad sobre el fraguado del MTA: estudio "in vitro" / The effect of moisture on the setting of MTA: an in vitro study

Objetivo: evaluar el efecto de la humedad sobre el fraguado del ProRoot MTA, en sus superficies intraconducto y apical. Materiales y métodos: se emplearon 20 probetas cilíndricas huecas, de silicona transparente, instaladas sobre un block de gomaespuma humedecido con suero fisiológico. Las probetas fueron obturadas con 4 mm de espesor de ProRoot MTA (Dentsply Tulsa Dental, OK, Estados Unidos) y divididas al azar en dos grupos de 10 probetas cada uno. En el grupo 1 se colocó sobre el material una torunda de algodón seco, mientras que en el grupo 2 el algodón estaba humedecido con agua destilada. Los orificios superiores de las probetas se obturaron con cemento de óxido de zinc reforzado Zoer´s (Laboratorio SL SA, Buenos Aires, Argentina). Los especímenes se mantuvieron a 37ºC de temperatura y 100% de humedad. A las 4 y 6 horas fueron removidos de la gomaespuma, se retiraron el Zoer´s y el algodón y se registró el fraguado en las superficies del MTA intraconducto -subgrupos 1A y 2A- y apical -subgrupos 1B y 2B-, empleando una aguja Gillmore. El procedimiento estadístico fue realizado mediante la prueba de Chi cuadrado. Resultados: a las 4 horas, se encontraba fraguado el 50 por ciento de las muestras del subgrupo 1A, el 80 por ciento de las del subgrupo 1B, el 90 por ciento de las del subgrupo 2A y el 100 por ciento de las del subgrupo 2B. A las 6 horas de control, todas las muestras se encontraban fraguadas (100 por ciento). Se registraron diferencias estadísticamente significativas -a las 4 horas- entre los subgrupos 1A y 2A (P<0,05); entre los subgrupos 1ºB y 2B no las hubo (P>0,05). Tampoco hubo diferencias estadísticamente significativas entre los subgrupos 1A y 1B ni entre los subgrupos 2A y 2B (P>0,05). Conclusión: la presencia de un algodón húmedo en contacto con el MTA acelera el fraguado de la superficie intraconducto del material, en tanto no influye en el fraguado de su superficie apical

Propriedades físico-químicas e biológicas de cimento experimental à base de Portland associado a diferentes radiopacificadores / Physical, chemical and biological properties of an experimental Portland-based cement with different radiopacifier agents

O objetivo do estudo foi avaliar as propriedades físicas, químicas e biológicas de um cimento experimental à base de Portland contendo diferentes agentes radiopacificadores. Os cimentos foram divididos em 6 grupos: 1. MTA-Angelus branco (controle), 2. cimento Portland branco (CP), 3. CP com 20% de óxido de zircônio (OZ), 4. CP/50% OZ, 5. CP com 20% de tungstato de cálcio (TC) e 6. CP/50% TC. As proporções foram determinadas em peso (80 e 50% de CP para 20 e 50% de radiopacificadores, respectivamente). Os cimentos experimentais foram manipulados utilizando 0,3 mL de líquido composto por 80% de água destilada e 20% de propilenoglicol, em volume. A caracterização dos materiais foi realizada em microscopia eletrônica de varredura (MEV), no modo EDS e em difratômetro de raios-X (XRD). Para os testes de radiopacidade, escoamento, espessura de filme e solubilidade foram seguidas as especificações no 57 da ANSI/ADA (2000). Na determinação do tempo de presa, foi empregada a norma ASTM C266/2008. Na análise do pH e liberação de íons cálcio foram analisados os períodos de 3, 24, 72 e 168 horas. Para análise da descoloração dentária, foram realizadas análises em espectrofotômetro, estereomicroscopia e MEV. Para análise da resposta inflamatória, foi utilizado o teste de implantação em subcutâneo de ratos. A utilização do óxido de zircônio e do tungstato de cálcio em combinação com o cimento Portland resultou no desenvolvimento de cimentos que exibiram uma radiopacidade próxima (20% de radiopacificadores) ou acima (50%) do recomendado pela norma no 57 da ANSI/ADA; tempo de presa mais prolongado, espessura de filme menor e escoamento mais elevado em comparação com o MTA-Angelus; solubilidade adequada e comparável ao MTA-Angelus, elevado pH e liberação de íons cálcio superior ao MTA-Angelus nos períodos iniciais e similar aos 7 dias, ausência de descoloração dentária e resposta inflamatória semelhante ao MTA-Angelus...

The aim of the study was to evaluate the physical, chemical and biological properties of an experimental calcium silicate-based cement with different concentrations of the radiopacifiers zirconium oxide and calcium tungstate. The materials were divided in 6 groups: 1. White MTA (control), 2. White Portland cement (PC), 3. PC with 20% zirconium oxide (ZO), 4. PC/50% ZO, 5. PC with 20% calcium tungstate (CT) and 6. PC/50% CT. The proportions were determined by weight. The cements were manipulated using 0.3 mL of liquid composed of 80% distilled water and 20% propylene glycol. The control was manipulated according to manufacturers instructions. The characterization of the materials was performed using scanning electron microscopy (SEM) in EDS mode and X-ray diffractometrer (XRD). For radiopacity, flowability, film thickness and solubility test, the specifications no 57 of ADA (2000) was followed. To determine the setting time, ASTM C266/2008 specifications was followed. For pH and calcium ion release assessment, the periods of 3, 24, 72 and 168 hours were evaluated. The analysis of dental discolouration was performed using spectrophotometer, stereomicroscopy and SEM. To evaluate the inflammatory response, the test of subcutaneous implantation in rats was used. The use of zirconium oxide and calcium tungstate in association with Portland cement resulted in development of cements with radiopacity nearly (20% radiopacifiers) or above (50%) the recommended by ANSI/ADA specifications n a 57, more prolonged setting time, lower film thickness and higher flowability in comparison with MTAAngelus, an adequate and comparable solubility with MTA-Angelus, high pH and calcium ion release higher than MTA-Angelus in the initial periods and similar at 7 days, absence of dental discolouration and inflammatory response similar to that presented by MTA-Angelus...

The influence of protective varnish on the integrity of orthodontic cements

OBJECTIVE: The aim of the present study was to assess the influence of saliva contamination over the structural strength and integrity of conventional glass-ionomer cements used for cementing orthodontic bands in the absence and presence of a surface-protecting varnish. METHOD: 48 samples were prepared by inserting 3 types of glass-ionomer cements into standardized metallic matrixes of 10 mm of diameter and 2 mm of depth. The cements used were: Meron (VOCO), Ketac-Cem (3M ESPE) and Vidrion C (DFL), all of which comprised groups A, B and C, respectively. Subgroups A1, B1 and C1 comprised samples with no surface protection, whereas subgroups A2, B2 and C2 comprised samples of which surface was coated with Cavitine varnish (SS White), after cement manipulation and application, in order to protect the cement applied. All samples were stored in artificial saliva for 24 hours at 37ºC. A Vickers diamond micro-durometer was used to produce indentations on the non-treated group (non-varnished) and the treated group (varnished). RESULTS: Varnished materials had significantly higher microhardness values in comparison to non-varnished materials. Ketac-Cem had the highest microhardness value among the varnished materials. CONCLUSION: Varnish application is necessary to preserve the cement and avoid enamel decalcification. Glass-ionomer cements should be protected in order to fully keep their properties, thus, contributing to dental health during orthodontic treatment.

OBJETIVO: avaliar a influência da contaminação salivar na resistência estrutural e integridade de cimentos de ionômero de vidro convencionais utilizados para cimentação de bandas ortodônticas na ausência e na presença de um verniz protetor de superfície. MÉTODOS: quarenta e oito corpos de prova foram confeccionados a partir de três cimentos ortodônticos, com auxílio de matrizes metálicas padronizadas com 10mm de diâmetro e 2mm de altura. Os cimentos utilizados foram: Meron (Voco), Ketac-Cem (3M ESPE) e Vidrion C (DFL), compondo os grupos A, B e C, respectivamente. Metade dessas amostras não recebeu nenhum tipo de proteção superficial, constituindo os subgrupos A1, B1 e C1, enquanto, os subgrupos A2, B2 e C2 tiveram suas superfícies isoladas com verniz Cavitine (SS White) após manipulação e aplicação do cimento, com intuito de proteger a superfície do cimento. As amostras foram armazenadas em saliva artificial por 24 horas a 37ºC. Foi realizado um ensaio de microdureza (Vickers) para avaliação da dureza de superfície do grupo não-tratado (sem isolamento) e do grupo tratado (agente protetor). RESULTADOS: os materiais previamente isolados com o verniz obtiveram valores de microdureza significativamente maiores que os não-isolados. O cimento Ketac-Cem apresentou, estatisticamente, a maior microdureza entre os materiais protegidos. CONCLUSÃO: o isolamento com verniz mostrou-se necessário para preservação do cimento e, consequentemente, de sua capacidade de evitar possíveis desmineralizações dentárias. Os cimentos de ionômero de vidro devem ser protegidos para manutenção de sua integridade, contribuindo para saúde dental durante o tratamento ortodôntico.

Anti-diabetic activity and safety assessment of Ayurvedic medicine, Jasada bhasma (zinc ash) in rats.

Jasada bhasma (zinc ash) is an extensively used Ayurvedic medicine for treating diabetes mellitus. The present communication presents yet unavailable comprehensive scientific data on its physico-chemical nature vis-à-vis anti-diabetic activity and toxicity profile.Zinc ash prepared by traditional method was found to consist of 200-500 nm sized particles, predominantly zinc oxide with hexagonal wurtzite crystal structure.The effective dose range of zinc ash in oral glucose tolerance tests performed using normoglycemic Wistar rats was found to be 3-30 mg/kg. Subsequently anti-diabetic activity was assessed in streptozotocin induced type 1 and type 2 diabetic rats. Four weeks treatment with zinc ash (1, 3, 10 mg/kg) resulted in improved glucose tolerance (16-19%), lowered blood glucose levels (20-33%) and reduced serum insulin levels (27-32%). Systemic absorption was assessed by single dose pharmacokinetic study where serum zinc levels were found to be elevated (3.5 folds) after oral administration of zinc ash. Acute and sub-acute toxicity tests demonstrated safety of zinc ash up to 300 mg/kg doseie. 100 times the efficacy dose in rats.These findings, the first of their kind, provide concrete scientific evidence that justifies usage of zinc ash in diabetes treatment.

Evaluation of pH, calcium ion release and antimicrobial activity of a new calcium aluminate cement

This study evaluated the pH, calcium ion release and antimicrobial activity of EndoBinder (EB), containing different radiopacifiers: bismuth oxide (Bi2O3), zinc oxide (ZnO) or zirconium oxide (ZrO2), in comparison to MTA. For pH and calcium ion release tests, 5 specimens per group (n = 5) were immersed into 10 mL of distilled and deionized water at 37°C. After 2, 4, 12, 24, 48 h; 7, 14 and 28 days, the pH was measured and calcium ion release quantified in an atomic absorption spectrophotometer. For antimicrobial activity, the cements were tested against S. aureus, E. coli, E. faecalis and C. albicans, in triplicate. MTA presented higher values for pH and calcium ion release than the other groups, however, with no statistically significant difference after 28 days (p > 0.05); and the largest inhibition halos for all strains, with no significant difference (E. coli and E. faecalis) for pure EB and EB + Bi2O3 (p > 0.05). EB presented similar performance to that of MTA as regards pH and calcium ion release; however, when ZnO and ZrO2 were used, EB did not present antimicrobial activity against some strains.

Evaluation of mechanical properties of five cements for orthodontic band cementation

The aim of this in vitro study was to compare the flexural, compressive and diametral tensile strengths of five cements used in orthodontics for band cementation. Twelve specimens of each cement were tested: 1 - GC Fuji Ortho Band (FJ), GC America Inc.; 2 - Meron (MR), Voco; 3 - Multi-Cure Glass Ionomer Band Cement (MC), 3M Unitek; 4 - Band-Lok (BL), Reliance Orthodontic Products; and 5 - Ketac Cem (KC), 3M ESPE. The results (mean) for diametral tensile strength were: 10.51 MPa (FJ), 9.60 MPa (MR), 20.04 MPa (MC), 42.80 MPa (BL), and 4.08 MPa (KC). The results for compressive strength were (in the same order): 64.50 MPa, 77.71 MPa, 94.21 MPa, 193.88 MPa, and 81.93 MPa. The results for flexural strength were (in the same order): 20.72 MPa, 25.84 MPa, 53.41 MPa, 137.41 MPa, and 20.50 MPa. The statistical analysis was performed by two-way ANOVA and Tukey tests with p-value £ 0.05. In terms of diametral tensile strength, BL showed the highest strength statistically, and MC, the second highest. In terms of compressive tensile strength, BL showed the highest strength statistically, and FJ did not attain the minimum recommended strength. In terms of flexural tensile strength, BL cement was superior to MC, and MR, FJ and KC were equivalent and inferior to BL and MC.

Effect of cement type and water storage time on the push-out bond strength of a glass fiber post

This study investigated the effects of the cement type and the water storage time on the push-out bond strength of a glass fiber post. Glass fiber posts (Fibrekor, Jeneric Pentron) were luted to post spaces using a self-cured resin cement (C&B Cement [CB]), a glass ionomer cement (Ketac Cem [KC]) or a resin-modified glass ionomer cement (GC FujiCEM [FC]) according to the manufacturers’ instructions. For each luting agent, the specimens were exposed to one of the following water storage times (n=5): 1 day (T1), 7 days (T7), 90 days (T90) and 180 days (T180). Push-out tests were performed after the storage times. Control specimens were not exposed to water storage, but subjected to the push-out test 10 min after post cementation. Data (in MPa) were analyzed by Kruskal-Wallis and Dunn`s test (α=0.05). Cement type and water storage time had a significant effect (p<0.05) on the push-out bond strength. CB showed significantly higher values of retention (p<0.05) than KC and FC, irrespective of the water storage time. Water storage increased significantly the push-out bond strength in T7 and T90, regardless of the cement type (p<0.05). The results showed that fiber posts luted to post spaces with the self-cured resin cement exhibited the best bonding performance throughout the 180-day water storage period. All cements exhibited a tendency to increase the bond strength after 7 and 90 days of water storage, decreasing thereafter.

Este estudo investigou os efeitos do tipo de cimento e do tempo de armazenamento em água na resistência adesiva ao cisalhamento de pinos de fibra. Pinos de fibra de vidro (Fibrekor, JenericPentron) foram cimentados aos condutos radiculares usando o cimento resinoso autopolimerizável (C&B Cement [CB]), o cimento de ionômero de vidro (Ketac Cem [KC]) ou o cimento de ionômero de vidro modificado por resina (GC FujiCEM [FC]) de acordo com as instruções dos fabricantes. Para cada cimento, os espécimes foram expostos a um dos seguintes tempos de armazenamento em água (n=5): 1 dia (T1), 7 dias (T7), 90 dias (T90) e 180 dias (T180). Os testes de resistência adesiva ao cisalhamento foram realizados após os períodos de armazenamento. Os espécimes controles não foram expostos ao armazenamento em água, mas submetidos ao teste de resistência adesiva ao cisalhamento 10 min após a cimentação do pino. Os dados (MPa) foram analisados usando Kruskal-Wallis e teste de Dunn (α=0,05). O tipo de cimento e o tempo de armazenamento em água afetaram significantemente a resistência adesiva ao cisalhamento (p<0,05). CB apresentou valores de retenção significantemente superiores a KC e FC, independentemente do tempo de armazenamento em água. Esse, por sua vez, aumentou significantemente a resistência adesiva ao cisalhamento em T7 e T90, independentemente do tipo de cimento (p<0,05). Os resultados indicaram que os pinos de fibra cimentados aos condutos radiculares com o cimento resinoso autopolimerizável apresentou a melhor performance adesiva durante todo o período de armazenamento em água de 180 dias. Todos os cimentos apresentaram uma tendência a aumentar a resistência adesiva ao cisalhamento após 7 e 90 dias de armazenamento em água, diminuindo posteriormente.

Efficacy of five rotary systems versus manual instrumentation during endodontic retreatment

The purpose of this study was to evaluate the efficiency of ProFile, GT, ProTaper, Race and K3 rotary instruments compared with hand K-files for removal of gutta-percha during retreatment. Sixty mandibular premolars were instrumented with GT rotary files and filled by thermomechanical compaction of gutta-percha and AH Plus sealer. The teeth are randomly divided into 6 groups of 10 specimens each. The roots were split longitudinally, digital images were created using a flatbed scanner, and the areas with remaining filling material were demarcated using Image Tool 1.21 software. The results indicate that GT left significantly less (p<0.05) remaining filling material (1.18 ± 1.47) than hand (3.70 ± 3.16) and Hero instruments (2.99 ± 2.58). There was no statistically significant difference (p<0.05) among the others techniques: ProFile (1.99 ± 2.66), ProTaper (2.00 ± 1.99) and K3 (2.71 ± 2.87) when compared with GT. In conclusion, GT, ProFile, ProTaper and K3 were more effective in removing gutta-percha than manual and Hero instruments.

O propósito deste estudo foi avaliar a eficiência dos instrumentos rotatórios ProFile, GT, ProTaper, Race e K3 comparados com limas K para a remoção do material obturador durante o retratamento. Sessenta pré-molares inferiores foram instrumentados com o sistema rotatório GT e obturados usando a técnica de compactação termomecânica de guta-percha com cimento AH Plus. Os dentes foram divididos aleatoriamente em seis grupos de dez espécimes cada. As raízes foram seccionadas longitudinalmente, imagens digitais foram criadas através de um scanner, e as áreas de remanescentes de material foram medidas usando o programa Image Tool 1.21. Os resultados indicaram que o GT deixou significantemente menos material remanescente (1,18 ± 1,47) do que os instrumentos manuais (3,70 ± 3,16) e os instrumentos Hero (2,99 ± 2,58) (p<0,05). Não houve diferença estatisticamente significante entre as outras técnicas ProFile (1,99 ± 2,66), ProTaper (2,00 ± 1,99) e K3 (2,71 ± 2,87) quando comparadas com o GT. Como conclusão, GT, ProFile, ProTaper e K3 foram revelados como mais efetivos em remover a guta-percha do que os instrumentos manuais e os instrumentos Hero.

Effect of temporary cements on the shear bond strength of luting cements

OBJECTIVE: The purpose of this study was to evaluate, by shear bond strength (SBS) testing, the influence of different types of temporary cements on the final cementation using conventional and self-etching resin-based luting cements. Material and Methods: Forty human teeth divided in two halves were assigned to 8 groups (n=10): I and V (no temporary cementation); II and VI: Ca(OH)2-based cement; III and VII: zinc oxide (ZO)-based cement; IV and VIII: ZO-eugenol (ZOE)-based cement. Final cementation was done with RelyX ARC cement (groups I to IV) and RelyX Unicem cement (groups V to VIII). Data were analyzed statistically by ANOVA and Tukey's test at 5 percent significance level. RESULTS: Means were (MPa): I - 3.80 (±1.481); II - 5.24 (±2.297); III - 6.98 (±1.885); IV - 6.54 (±1.459); V - 5.22 (±2.465); VI - 4.48 (±1.705); VII - 6.29 (±2.280); VIII - 2.47 (±2.076). Comparison of the groups that had the same temporary cementation (Groups II and VI; III and VII; IV and VIII) showed statistically significant difference (p<0.001) only between Groups IV and VIII, in which ZOE-based cements were used. The use of either Ca(OH)2-based (Groups II and VI) or ZO-based (Groups III and VII) cements showed no statistically significant difference (p>0.05) for the different luting cements (RelyX TM ARC and RelyX TM Unicem). The groups that had no temporary cementation (Groups I and V) did not differ significantly from each other either (p>0.05). CONCLUSION: When temporary cementation was done with ZO- or ZOE-based cements and final cementation was done with RelyX ARC, there was an increase in the SBS compared to the control. In the groups cemented with RelyX Unicem, however, the use of a ZOE-based temporary cement affected negatively the SBS of the luting agent used for final cementation.

Influence of serum and necrotic soft tissue on the antimicrobial effects of intracanal medicaments

The purpose of this study was to investigate the influence of serum and necrotic soft tissue on the antimicrobial activity of intracanal medicaments. The medicaments tested were: calcium hydroxyde/glycerin paste, calcium hydroxide/chlorhexidine paste, calcium hydroxide/camphorated paramonochlorophenol/glycerin paste, and chlorhexidine/zinc oxide paste. Survival of Enterococcus faecalis and Candida albicans exposed to the medicaments tested in the presence or absence of serum or necrotic tissue was monitored in three in vitro experiments where samples for culturing were taken at different time periods. The overall results demonstrated that the antimicrobial activity of all intracanal medicaments tested was slowed down in the presence of necrotic tissue. Calcium hydroxide pastes in glycerin or chlorhexidine were significantly affected by serum. Of the medicaments tested in this study, the least affected was the calcium hydroxide/camphorated paramonochlorophenol/glycerin paste.

O objetivo deste estudo foi avaliar a influência do soro e de tecido mole necrosado na atividade antimicrobiana de medicamentos intra-canais. Os medicamentos testados foram pastas de hidróxido de cálcio/glicerina, hidróxido de cálcio/clorexidina, hidróxido de cálcio/paramonoclorofenol canforado/glicerina e clorexidina/óxido de zinco. A sobrevivência de Enterococcus faecalis e Candida albicans expostos aos medicamentos na presença ou ausência de soro ou tecido necrosado foi monitorada em três experimentos in vitro nos quais amostras para cultura foram avaliadas em diferentes períodos de tempo. No geral, os resultados demonstraram que a atividade antimicrobiana de todos os medicamentos testados foi retardada na presença de soro ou de tecido necrosado. As pastas de hidróxido de cálcio em glicerina ou clorexidina foram significativamente afetadas pelo soro. Dos medicamentos testados, o menos afetado foi a pasta de hidróxido de cálcio/paramonoclorofenol canforado/glicerina.

Biological compatibility of the endodontic paste prepared with tetracycline, thiamphenicol and zinc oxide implanted on the subcutaneous tissue of rats / Compatibilidad biológica de pasta endodóntica preparada con tetraciclina,tiamfenicol y óxido de zinc, implantado en el tejido subcutáneo de ratas

An important requirement for endodontic paste with antibiotics placed in direct contact with living tissues is biocompatibility. The aim of this study was to evaluate the paste biocompatibility prepared with zinc oxide (1.25mg), tetracycline (8mg) and thiamphenicol (26.67mg). The paste and its components were implanted separately through polyethylene sterile tubes of 10mm in length and 1.3mm in diameter, in the subcutaneous tissue of rats with the experiment control at intervals of 3, 7, 15 and 30 days. Each day 6 rats were used, being 3 of them with implant of the substances in four sites placed on the back of the animals and 3 sham animals where it was implanted the polyethylene empty tubes. The experimental animals were anesthetized in an intra-peritoneal way with ketamina and xilazina (0.75ml / g body weight). After the experimental periods, the animals were anesthetized with the same anesthetic overdose. It was held an excision biopsy of the implant area with 10 mm to the security limit included in paraffin following a plan of random histological cut and uniformlyisotropic or oriented cuts according to stereological principles, getting a statistical estimative of the relative amount of inflammatory cells or not on the test system, getting as a result the paste biocompatibility, being the zinc oxide the most toxic element for the cell quality found.

Un requisito importante para la pasta endodóntica preparada con antibióticos, que es colocada en contacto directo con los tejidos vivos es la biocompatibilidad. El objetivo de este estudio fue evaluar la biocompatibilidad de la pasta preparada con óxido del zinc (1,25mg), tetraciclina (8mg) y el tiamfenicol (26,67mg). La pasta y sus componentes fueron implantados por separado a través de tubos estériles de polietileno de 10 mm de longitud y de 1,3mm de diámetro en el tejido subcutáneo de ratas en intervalos de 3, 7, 15 y 30 días. Cada día, 6 ratas fueron implantadas en cuatro sitios ubicados en la parte posterior de los animales, 3 de ellas con el implante de las sustancias y 3 fueron implantados con los tubos de polietileno vacíos. Los animales del experimento fueron anestesiados intraperitteonealmen, con ketamina y xilasina (0,75 ml/g peso corporal). Después de los periodos experimentales, los animales fueron anestesiados con la misma sobredosis anestésica. Fue realizada una biopsia exisional del área del implante con 10 mm de límite de seguridad, luego se realizaron cortes histológico al azar uniformemente isotrópicos o orientados según los principios esteriológicos, consiguiendo un estimativo estadístico de la cantidad relativa de células inflamatorias en el sistema de prueba. Se obtuvieron resultados de la biocompatibilidad de la pasta, siendo el óxido del zinc el elemento mas tóxico según la cualidad de las células que fueron encontradas.